RT Journal Article
SR Electronic
T1 Generation and assembly of secretory antibodies in plants
JF Science
JO Science
FD American Association for the Advancement of Science
SP 716
OP 719
DO 10.1126/science.7732380
VO 268
IS 5211
A1 Ma, JK
A1 Hiatt, A
A1 Hein, M
A1 Vine, ND
A1 Wang, F
A1 Stabila, P
A1 van Dolleweerd, C
A1 Mostov, K
A1 Lehner, T
YR 1995
UL http://science.sciencemag.org/content/268/5211/716.abstract
AB Four transgenic Nicotiana tabacum plants were generated that expressed a murine monoclonal antibody kappa chain, a hybrid immunoglobulin A-G heavy chain, a murine joining chain, and a rabbit secretory component, respectively. Successive sexual crosses between these plants and filial recombinants resulted in plants that expressed all four protein chains simultaneously. These chains were assembled into a functional, high molecular weight secretory immunoglobulin that recognized the native streptococcal antigen I/II cell surface adhesion molecule. In plants, single cells are able to assemble secretory antibodies, whereas two different cell types are required in mammals. Transgenic plants may be suitable for large-scale production of recombinant secretory immunoglobulin A for passive mucosal immunotherapy. Plant cells also possess the requisite mechanisms for assembly and expression of other complex recombinant protein molecules.