PT  - JOURNAL ARTICLE
AU  - Allen, GJ
AU  - Muir, 
AU  - Sanders, D
TI  - Release of Ca2+ from individual plant vacuoles by both InsP3 and cyclic ADP-ribose
AID  - 10.1126/science.7732384
DP  - 1995 May 05
TA  - Science
PG  - 735--737
VI  - 268
IP  - 5211
4099  - http://science.sciencemag.org/content/268/5211/735.short
4100  - http://science.sciencemag.org/content/268/5211/735.full
SO  - Science1995 May 05; 268
AB  - Calcium mobilization from intracellular pools couples many stimuli to responses in plant cells. Cyclic adenosine 5'-diphosphoribose (cADPR), which interacts with a ryanodine receptor in certain animal cells, was shown to elicit calcium release at the vacuolar membrane of beet storage root. The vacuolar calcium release pathway showed similarities to cADPR-gated calcium release in animal cells, including inhibition by ruthenium red, ryanodine activation, and high affinity for cADPR [Michaelis constant (Km) = 24 +/- 7 nanomolar]. Analysis by patch-clamping demonstrated that the cADPR-gated pathway in beet is voltage-dependent over the physiological range, does not spontaneously desensitize, and is colocalized with an inositol 1,4,5-trisphosphate (InsP3)-gated calcium release pathway in individual vacuoles.