RT Journal Article
SR Electronic
T1 Association of flavin adenine dinucleotide with the Arabidopsis blue light receptor CRY1
JF Science
JO Science
FD American Association for the Advancement of Science
SP 968
OP 970
DO 10.1126/science.7638620
VO 269
IS 5226
A1 Lin, C
A1 Robertson, DE
A1 Ahmad, M
A1 Raibekas, AA
A1 Jorns, MS
A1 Dutton, PL
A1 Cashmore, AR
YR 1995
UL http://science.sciencemag.org/content/269/5226/968.abstract
AB The arabidopsis thaliana HY4 gene encodes CRY1, a 75-kilodalton flavoprotein mediating blue light-dependent regulation of seedling development. CRY1 is demonstrated here to noncovalently bind stoichiometric amounts of flavin adenine dinucleotide (FAD). The redox properties of FAD bound by CRY1 include an unexpected stability of the neutral radical flavosemiquinone (FADH.). The absorption properties of this flavosemiquinone provide a likely explanation for the additional sensitivity exhibited by CRY1-mediated responses in the green region of the visible spectrum. Despite the sequence homology to microbial DNA photolyases, CRY1 was found to have no detectable photolyase activity.