PT - JOURNAL ARTICLE AU - Kuhn, R AU - Schwenk, F AU - Aguet, M AU - Rajewsky, K TI - Inducible gene targeting in mice AID - 10.1126/science.7660125 DP - 1995 Sep 08 TA - Science PG - 1427--1429 VI - 269 IP - 5229 4099 - http://science.sciencemag.org/content/269/5229/1427.short 4100 - http://science.sciencemag.org/content/269/5229/1427.full SO - Science1995 Sep 08; 269 AB - A method of gene targeting that allows the inducible inactivation of a target gene in mice is presented. The method uses an interferon-responsive promoter to control the expression of Cre recombinase. Here, Cre was used to delete a segment of the DNA polymerase beta gene flanked by IoxP recombinase recognition sites. Deletion was complete in liver and nearly complete in lymphocytes within a few days, whereas partial deletion was obtained in other tissues. This method can be used for the inducible inactivation of any other gene in vivo.