RT Journal Article
SR Electronic
T1 Requirement for Generation of H<sub>2</sub>O<sub>2</sub> for Platelet-Derived Growth Factor Signal Transduction
JF Science
JO Science
FD American Association for the Advancement of Science
SP 296
OP 299
DO 10.1126/science.270.5234.296
VO 270
IS 5234
A1 Sundaresan, Maitrayee
A1 Yu, Zu-Xi
A1 Ferrans, Victor J.
A1 Irani, Kaikobad
A1 Finkel, Toren
YR 1995
UL http://science.sciencemag.org/content/270/5234/296.abstract
AB Stimulation of rat vascular smooth muscle cells (VSMCs) by platelet-derived growth factor (PDGF) transiently increased the intracellular concentration of hydrogen peroxide (H2O2). This increase could be blunted by increasing the intracellular concentration of the scavenging enzyme catalase or by the chemical antioxidant N-acetylcysteine. The response of VSMCs to PDGF, which includes tyrosine phosphorylation, mitogen-activated protein kinase stimulation, DNA synthesis, and chemotaxis, was inhibited when the growth factor-stimulated rise in H2O2 concentration was blocked. These results suggest that H2O2 may act as a signal-transducing molecule, and they suggest a potential mechanism for the cardioprotective effects of antioxidants.