RT Journal Article
SR Electronic
T1 Attenuated <em>Shigella</em> as a DNA Delivery Vehicle for DNA-Mediated Immunization
JF Science
JO Science
FD American Association for the Advancement of Science
SP 299
OP 303
DO 10.1126/science.270.5234.299
VO 270
IS 5234
A1 Sizemore, Donata R.
A1 Branstrom, Arthur A.
A1 Sadoff, Jerald C.
YR 1995
UL http://science.sciencemag.org/content/270/5234/299.abstract
AB Direct inoculation of DNA, in the form of purified bacterial plasmids that are unable to replicate in mammalian cells but are able to direct cell synthesis of foreign proteins, is being explored as an approach to vaccine development. Here, a highly attenuated Shigella vector invaded mammalian cells and delivered such plasmids into the cytoplasm of cells, and subsequent production of functional foreign protein was measured. Because this Shigella vector was designed to deliver DNA to colonic mucosa, the method is a potential basis for oral and other mucosal DNA immunization and gene therapy strategies.