RT Journal Article
SR Electronic
T1 Myt1: A Membrane-Associated Inhibitory Kinase That Phosphorylates Cdc2 on Both Threonine-14 and Tyrosine-15
JF Science
JO Science
FD American Association for the Advancement of Science
SP 86
OP 90
DO 10.1126/science.270.5233.86
VO 270
IS 5233
A1 Mueller, Paul R.
A1 Coleman, Thomas R.
A1 Kumagai, Akiko
A1 Dunphy, William G.
YR 1995
UL http://science.sciencemag.org/content/270/5233/86.abstract
AB Cdc2 is the cyclin-dependent kinase that controls entry of cells into mitosis. Phospho-rylation of Cdc2 on threonine-14 and tyrosine-15 inhibits the activity of the enzyme and prevents premature initiation of mitosis. Although Wee1 has been identified as the kinase that phosphorylates tyrosine-15 in various organisms, the threonine-14-specific kinase has not been isolated. A complementary DNA was cloned from Xenopus that encodes Myt1, a member of the Wee1 family that was discovered to phosphorylate Cdc2 efficiently on both threonine-14 and tyrosine-15. Myt1 is a membrane-associated protein that contains a putative transmembrane segment. Immunodepletion studies suggested that Myt1 is the predominant threonine-14-specific kinase in Xenopus egg extracts. Myt1 activity is highly regulated during the cell cycle, suggesting that this relative of Wee1 plays a role in mitotic control.