PT  - JOURNAL ARTICLE
AU  - Schena, Mark
AU  - Shalon, Dari
AU  - Davis, Ronald W.
AU  - Brown, Patrick O.
TI  - Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
AID  - 10.1126/science.270.5235.467
DP  - 1995 Oct 20
TA  - Science
PG  - 467--470
VI  - 270
IP  - 5235
4099  - http://science.sciencemag.org/content/270/5235/467.short
4100  - http://science.sciencemag.org/content/270/5235/467.full
SO  - Science1995 Oct 20; 270
AB  - A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.