PT - JOURNAL ARTICLE AU - Schena, Mark AU - Shalon, Dari AU - Davis, Ronald W. AU - Brown, Patrick O. TI - Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray AID - 10.1126/science.270.5235.467 DP - 1995 Oct 20 TA - Science PG - 467--470 VI - 270 IP - 5235 4099 - http://science.sciencemag.org/content/270/5235/467.short 4100 - http://science.sciencemag.org/content/270/5235/467.full SO - Science1995 Oct 20; 270 AB - A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.