PT  - JOURNAL ARTICLE
AU  - Fukasawa, Kenji
AU  - Choi, Taesaeng
AU  - Kuriyama, Ryoko
AU  - Rulong, Shen
AU  - Woude, George F. Vande
TI  - Abnormal Centrosome Amplification in the Absence of p53
AID  - 10.1126/science.271.5256.1744
DP  - 1996 Mar 22
TA  - Science
PG  - 1744--1747
VI  - 271
IP  - 5256
4099  - http://science.sciencemag.org/content/271/5256/1744.short
4100  - http://science.sciencemag.org/content/271/5256/1744.full
SO  - Science1996 Mar 22; 271
AB  - The centrosome plays a vital role in mitotic fidelity, ensuring establishment of bipolar spindles and balanced chromosome segregation. Centrosome duplication occurs only once during the cell cycle and is therefore highly regulated. Here, it is shown that in mouse embryonic fibroblasts (MEFs) lacking the p53 tumor suppressor protein, multiple copies of functionally competent centrosomes are generated during a single cell cycle. In contrast, MEFs prepared from normal mice or mice deficient in the retinoblastoma tumor suppressor gene product do not display these abnormalities. The abnormally amplified centrosomes profoundly affect mitotic fidelity, resulting in unequal segregation of chromosomes. These observations implicate p53 in the regulation of centrosome duplication and suggest one possible mechanism by which the loss of p53 may cause genetic instability.