RT Journal Article
SR Electronic
T1 Identification of Subunits of the Anaphase-Promoting Complex of <em>Saccharomyces cerevisiae</em>
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1201
OP 1204
DO 10.1126/science.274.5290.1201
VO 274
IS 5290
A1 Zachariae, Wolfgang
A1 Shin, Tae Ho
A1 Galova, Marta
A1 Obermaier, Brigitte
A1 Nasmyth, Kim
YR 1996
UL http://science.sciencemag.org/content/274/5290/1201.abstract
AB Entry into anaphase and proteolysis of B-type cyclins depend on a complex containing the tetratricopeptide repeat proteins Cdc16p, Cdc23p, and Cdc27p. This particle, called the anaphase-promoting complex (APC) or cyclosome, functions as a cell cycle-regulated ubiquitin-protein ligase. Two additional subunits of the budding yeast APC were identified: The largest subunit, encoded by the APC1 gene, is conserved between fungi and vertebrates and shows similarity to BIMEp from Aspergillus nidulans. A small heat-inducible subunit is encoded by the CDC26 gene. The yeast APC is a 36S particle that contains at least seven different proteins.