PT  - JOURNAL ARTICLE
AU  - Ulmasov, Tim
AU  - Hagen, Gretchen
AU  - Guilfoyle, Tom J.
TI  - ARF1, a Transcription Factor That Binds to Auxin Response Elements
AID  - 10.1126/science.276.5320.1865
DP  - 1997 Jun 20
TA  - Science
PG  - 1865--1868
VI  - 276
IP  - 5320
4099  - http://science.sciencemag.org/content/276/5320/1865.short
4100  - http://science.sciencemag.org/content/276/5320/1865.full
SO  - Science1997 Jun 20; 276
AB  - The plant hormone auxin regulates plant physiology by modulating the interaction of transcription factors with auxin response elements (AuxREs) of the affected genes. A transcription factor, Auxin Response Factor 1 (ARF1), that binds to the sequence TGTCTC in AuxREs was cloned from Arabidopsis by using a yeast one-hybrid system. ARF1 has an amino-terminal DNA-binding domain related to the carboxyl terminus of the maize transactivator Viviparous-1. Sequence requirements for ARF1 binding in vitro are identical to those that confer auxin responsiveness in vivo. The carboxyl terminus of ARF1 contains two motifs found in the Aux/IAA class of proteins and appears to mediate protein-protein interactions.