PT  - JOURNAL ARTICLE
AU  - Hordijk, Peter L.
AU  - ten Klooster, Jean Paul
AU  - van der Kammen, Rob A.
AU  - Michiels, Frits
AU  - Oomen, Lauran C. J. M.
AU  - Collard, John G.
TI  - Inhibition of Invasion of Epithelial Cells by Tiam1-Rac Signaling
AID  - 10.1126/science.278.5342.1464
DP  - 1997 Nov 21
TA  - Science
PG  - 1464--1466
VI  - 278
IP  - 5342
4099  - http://science.sciencemag.org/content/278/5342/1464.short
4100  - http://science.sciencemag.org/content/278/5342/1464.full
SO  - Science1997 Nov 21; 278
AB  - Tiam1 encodes an exchange factor for the Rho-like guanosine triphosphatase Rac. Both Tiam1 and activated RacV12 promote invasiveness of T lymphoma cells. In epithelial Madin–Darby canine kidney (MDCK) cells, Tiam1 localized to adherens junctions. Ectopic expression of Tiam1 or RacV12 inhibited hepatocyte growth factor–induced scattering by increasing E-cadherin–mediated cell-cell adhesion accompanied by actin polymerization at cell-cell contacts. In Ras-transformed MDCK cells, expression of Tiam1 or RacV12 restored E-cadherin–mediated adhesion, resulting in phenotypic reversion and loss of invasiveness. These data suggest an invasion-suppressor role for Tiam1 and Rac in epithelial cells.