PT  - JOURNAL ARTICLE
AU  - Kim, Sang Hoon
AU  - Lin, Diana P.
AU  - Matsumoto, Sanae
AU  - Kitazono, Ana
AU  - Matsumoto, Tomohiro
TI  - Fission Yeast Slp1: An Effector of the Mad2-Dependent Spindle Checkpoint
AID  - 10.1126/science.279.5353.1045
DP  - 1998 Feb 13
TA  - Science
PG  - 1045--1047
VI  - 279
IP  - 5353
4099  - http://science.sciencemag.org/content/279/5353/1045.short
4100  - http://science.sciencemag.org/content/279/5353/1045.full
SO  - Science1998 Feb 13; 279
AB  - Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan–aspartic acid)–repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.