PT - JOURNAL ARTICLE AU - Kim, Sang Hoon AU - Lin, Diana P. AU - Matsumoto, Sanae AU - Kitazono, Ana AU - Matsumoto, Tomohiro TI - Fission Yeast Slp1: An Effector of the Mad2-Dependent Spindle Checkpoint AID - 10.1126/science.279.5353.1045 DP - 1998 Feb 13 TA - Science PG - 1045--1047 VI - 279 IP - 5353 4099 - http://science.sciencemag.org/content/279/5353/1045.short 4100 - http://science.sciencemag.org/content/279/5353/1045.full SO - Science1998 Feb 13; 279 AB - Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan–aspartic acid)–repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.