RT Journal Article
SR Electronic
T1 Fission Yeast Slp1: An Effector of the Mad2-Dependent Spindle Checkpoint
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1045
OP 1047
DO 10.1126/science.279.5353.1045
VO 279
IS 5353
A1 Kim, Sang Hoon
A1 Lin, Diana P.
A1 Matsumoto, Sanae
A1 Kitazono, Ana
A1 Matsumoto, Tomohiro
YR 1998
UL http://science.sciencemag.org/content/279/5353/1045.abstract
AB Mad2 is a component of the spindle checkpoint, which delays the onset of anaphase until all chromosomes are attached to the spindle. Mad2 formed a complex with Slp1, a WD (tryptophan–aspartic acid)–repeat protein essential for the onset of anaphase. When the physical interaction between the two proteins was disrupted, the spindle checkpoint was no longer functional. Post-anaphase events such as chromosome decondensation and the next round of DNA replication were not delayed even when the spindle assembly was incomplete. This relief of dependence appears to be a result of deregulation of ubiquitin-dependent proteolysis mediated by the anaphase-promoting complex.