RT Journal Article
SR Electronic
T1 Abnormal Spindle Protein, Asp, and the Integrity of Mitotic Centrosomal Microtubule Organizing Centers
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1733
OP 1735
DO 10.1126/science.283.5408.1733
VO 283
IS 5408
A1 do Carmo Avides, Maria
A1 Glover, David M.
YR 1999
UL http://science.sciencemag.org/content/283/5408/1733.abstract
AB The product of the abnormal spindle (asp) gene was found to be an asymmetrically localized component of the centrosome during mitosis, required to focus the poles of the mitotic spindle in vivo. Removing Asp protein function from Drosophila melanogaster embryo extracts, either by mutation or immunodepletion, resulted in loss of their ability to restore microtubule-organizing center activity to salt-stripped centrosome preparations. This was corrected by addition of purified Asp protein. Thus, Asp appears to hold together the microtubule-nucleating γ-tubulin ring complexes that organize the mitotic centrosome.