RT Journal Article
SR Electronic
T1 A Biochemical Genomics Approach for Identifying Genes by the Activity of Their Products
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1153
OP 1155
DO 10.1126/science.286.5442.1153
VO 286
IS 5442
A1 Martzen, Mark R.
A1 McCraith, Stephen M.
A1 Spinelli, Sherry L.
A1 Torres, Francy M.
A1 Fields, Stanley
A1 Grayhack, Elizabeth J.
A1 Phizicky, Eric M.
YR 1999
UL http://science.sciencemag.org/content/286/5442/1153.abstract
AB For the identification of yeast genes specifying biochemical activities, a genomic strategy that is rapid, sensitive, and widely applicable was developed with an array of 6144 individual yeast strains, each containing a different yeast open reading frame (ORF) fused to glutathione S-transferase (GST). For the identification of ORF-associated activities, strains were grown in defined pools, and GST-ORFs were purified. Then, pools were assayed for activities, and active pools were deconvoluted to identify the source strains. Three previously unknown ORF-associated activities were identified with this strategy: a cyclic phosphodiesterase that acts on adenosine diphosphate–ribose 1"-2" cyclic phosphate (Appr>p), an Appr-1"-p–processing activity, and a cytochrome c methyltransferase.