RT Journal Article
SR Electronic
T1 Genetic Definition and Sequence Analysis of <em>Arabidopsis</em> Centromeres
JF Science
JO Science
FD American Association for the Advancement of Science
SP 2468
OP 2474
DO 10.1126/science.286.5449.2468
VO 286
IS 5449
A1 Copenhaver, Gregory P.
A1 Nickel, Kathryn
A1 Kuromori, Takashi
A1 Benito, Maria-Ines
A1 Kaul, Samir
A1 Lin, Xiaoying
A1 Bevan, Michael
A1 Murphy, George
A1 Harris, Barbara
A1 Parnell, Laurence D.
A1 McCombie, W. Richard
A1 Martienssen, Robert A.
A1 Marra, Marco
A1 Preuss, Daphne
YR 1999
UL http://science.sciencemag.org/content/286/5449/2468.abstract
AB High-precision genetic mapping was used to define the regions that contain centromere functions on each natural chromosome inArabidopsis thaliana. These regions exhibited dramatic recombinational repression and contained complex DNA surrounding large arrays of 180–base pair repeats. Unexpectedly, the DNA within the centromeres was not merely structural but also encoded several expressed genes. The regions flanking the centromeres were densely populated by repetitive elements yet experienced normal levels of recombination. The genetically defined centromeres were well conserved among Arabidopsis ecotypes but displayed limited sequence homology between different chromosomes, excluding repetitive DNA. This investigation provides a platform for dissecting the role of individual sequences in centromeres in higher eukaryotes.