RT Journal Article
SR Electronic
T1 Structure of the Cytoplasmic β Subunit--T1 Assembly of Voltage-Dependent K<sup>+</sup> Channels
JF Science
JO Science
FD American Association for the Advancement of Science
SP 123
OP 127
DO 10.1126/science.289.5476.123
VO 289
IS 5476
A1 Gulbis, Jacqueline M.
A1 Zhou, Ming
A1 Mann, Sabine
A1 MacKinnon, Roderick
YR 2000
UL http://science.sciencemag.org/content/289/5476/123.abstract
AB The structure of the cytoplasmic assembly of voltage-dependent K+ channels was solved by x-ray crystallography at 2.1 angstrom resolution. The assembly includes the cytoplasmic (T1) domain of the integral membrane α subunit together with the oxidoreductase β subunit in a fourfold symmetric T14β4 complex. An electrophysiological assay showed that this complex is oriented with four T1 domains facing the transmembrane pore and four β subunits facing the cytoplasm. The transmembrane pore communicates with the cytoplasm through lateral, negatively charged openings above the T14β4complex. The inactivation peptides of voltage-dependent K+channels reach their site of action by entering these openings.