RT Journal Article SR Electronic T1 Genome-Wide Location and Function of DNA Binding Proteins JF Science JO Science FD American Association for the Advancement of Science SP 2306 OP 2309 DO 10.1126/science.290.5500.2306 VO 290 IS 5500 A1 Ren, Bing A1 Robert, François A1 Wyrick, John J. A1 Aparicio, Oscar A1 Jennings, Ezra G. A1 Simon, Itamar A1 Zeitlinger, Julia A1 Schreiber, Jörg A1 Hannett, Nancy A1 Kanin, Elenita A1 Volkert, Thomas L. A1 Wilson, Christopher J. A1 Bell, Stephen P. A1 Young, Richard A. YR 2000 UL http://science.sciencemag.org/content/290/5500/2306.abstract AB Understanding how DNA binding proteins control global gene expression and chromosomal maintenance requires knowledge of the chromosomal locations at which these proteins function in vivo. We developed a microarray method that reveals the genome-wide location of DNA-bound proteins and used this method to monitor binding of gene-specific transcription activators in yeast. A combination of location and expression profiles was used to identify genes whose expression is directly controlled by Gal4 and Ste12 as cells respond to changes in carbon source and mating pheromone, respectively. The results identify pathways that are coordinately regulated by each of the two activators and reveal previously unknown functions for Gal4 and Ste12. Genome-wide location analysis will facilitate investigation of gene regulatory networks, gene function, and genome maintenance.