RT Journal Article
SR Electronic
T1 Genome-Wide Location and Function of DNA Binding Proteins
JF Science
JO Science
FD American Association for the Advancement of Science
SP 2306
OP 2309
DO 10.1126/science.290.5500.2306
VO 290
IS 5500
A1 Ren, Bing
A1 Robert, François
A1 Wyrick, John J.
A1 Aparicio, Oscar
A1 Jennings, Ezra G.
A1 Simon, Itamar
A1 Zeitlinger, Julia
A1 Schreiber, Jörg
A1 Hannett, Nancy
A1 Kanin, Elenita
A1 Volkert, Thomas L.
A1 Wilson, Christopher J.
A1 Bell, Stephen P.
A1 Young, Richard A.
YR 2000
UL http://science.sciencemag.org/content/290/5500/2306.abstract
AB Understanding how DNA binding proteins control global gene expression and chromosomal maintenance requires knowledge of the chromosomal locations at which these proteins function in vivo. We developed a microarray method that reveals the genome-wide location of DNA-bound proteins and used this method to monitor binding of gene-specific transcription activators in yeast. A combination of location and expression profiles was used to identify genes whose expression is directly controlled by Gal4 and Ste12 as cells respond to changes in carbon source and mating pheromone, respectively. The results identify pathways that are coordinately regulated by each of the two activators and reveal previously unknown functions for Gal4 and Ste12. Genome-wide location analysis will facilitate investigation of gene regulatory networks, gene function, and genome maintenance.