PT  - JOURNAL ARTICLE
AU  - Runnels, Loren W.
AU  - Yue, Lixia
AU  - Clapham, David E.
TI  - TRP-PLIK, a Bifunctional Protein with Kinase and Ion Channel Activities
AID  - 10.1126/science.1058519
DP  - 2001 Feb 09
TA  - Science
PG  - 1043--1047
VI  - 291
IP  - 5506
4099  - http://science.sciencemag.org/content/291/5506/1043.short
4100  - http://science.sciencemag.org/content/291/5506/1043.full
SO  - Science2001 Feb 09; 291
AB  - We cloned and characterized a protein kinase and ion channel, TRP-PLIK. As part of the long transient receptor potential channel subfamily implicated in control of cell division, it is a protein that is both an ion channel and a protein kinase. TRP-PLIK phosphorylated itself, displayed a wide tissue distribution, and, when expressed in CHO-K1 cells, constituted a nonselective, calcium-permeant, 105-picosiemen, steeply outwardly rectifying conductance. The zinc finger containing α-kinase domain was functional. Inactivation of the kinase activity by site-directed mutagenesis and the channel's dependence on intracellular adenosine triphosphate (ATP) demonstrated that the channel's kinase activity is essential for channel function.