PT  - JOURNAL ARTICLE
AU  - Sharp, Marc D.
AU  - Pogliano, Kit
TI  - Role of Cell-Specific SpoIIIE Assembly in Polarity of DNA Transfer
AID  - 10.1126/science.1066274
DP  - 2002 Jan 04
TA  - Science
PG  - 137--139
VI  - 295
IP  - 5552
4099  - http://science.sciencemag.org/content/295/5552/137.short
4100  - http://science.sciencemag.org/content/295/5552/137.full
SO  - Science2002 Jan 04; 295
AB  - SpoIIIE mediates postseptational chromosome partitioning in Bacillus subtilis, but the mechanism controlling the direction of DNA transfer remains obscure. Here, we demonstrated that SpoIIIE acts as a DNA exporter: When SpoIIIE was synthesized in the larger of the two cells necessary for sporulation, the mother cell, DNA was translocated into the smaller forespore; however, when it was synthesized in the forespore, DNA was translocated into the mother cell. Furthermore, the DNA-tracking domain of SpoIIIE inhibited SpoIIIE complex assembly in the forespore. Thus, during sporulation, chromosome partitioning is controlled by the preferential assembly of SpoIIIE in one daughter cell.