RT Journal Article
SR Electronic
T1 Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors
JF Science
JO Science
FD American Association for the Advancement of Science
SP 868
OP 872
DO 10.1126/science.1067081
VO 295
IS 5556
A1 Lois, Carlos
A1 Hong, Elizabeth J.
A1 Pease, Shirley
A1 Brown, Eric J.
A1 Baltimore, David
YR 2002
UL http://science.sciencemag.org/content/295/5556/868.abstract
AB Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte–specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.