RT Journal Article
SR Electronic
T1 Retrograde Support of Neuronal Survival Without Retrograde Transport of Nerve Growth Factor
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1536
OP 1539
DO 10.1126/science.1064913
VO 295
IS 5559
A1 MacInnis, Bronwyn L.
A1 Campenot, Robert B.
YR 2002
UL http://science.sciencemag.org/content/295/5559/1536.abstract
AB Application of nerve growth factor (NGF) covalently cross-linked to beads increased the phosphorylation of TrkA and Akt, but not of mitogen-activated protein kinase, in cultured rat sympathetic neurons. NGF beads or iodine-125–labeled NGF beads supplied to distal axons resulted in the survival of over 80% of the neurons for 30 hours, with little or no retrograde transport of iodine-125–labeled NGF; whereas application of free iodine-125–labeled NGF (0.5 nanograms per milliliter) produced 20-fold more retrograde transport, but only 29% of the neurons survived. Thus, in contrast to widely accepted theory, a neuronal survival signal can reach the cell bodies unaccompanied by the NGF that initiated it.