PT  - JOURNAL ARTICLE
AU  - Seki, Motoaki
AU  - Narusaka, Mari
AU  - Kamiya, Asako
AU  - Ishida, Junko
AU  - Satou, Masakazu
AU  - Sakurai, Tetsuya
AU  - Nakajima, Maiko
AU  - Enju, Akiko
AU  - Akiyama, Kenji
AU  - Oono, Youko
AU  - Muramatsu, Masami
AU  - Hayashizaki, Yoshihide
AU  - Kawai, Jun
AU  - Carninci, Piero
AU  - Itoh, Masayoshi
AU  - Ishii, Yoshiyuki
AU  - Arakawa, Takahiro
AU  - Shibata, Kazuhiro
AU  - Shinagawa, Akira
AU  - Shinozaki, Kazuo
TI  - Functional Annotation of a Full-Length <em>Arabidopsis </em>cDNA Collection
AID  - 10.1126/science.1071006
DP  - 2002 Apr 05
TA  - Science
PG  - 141--145
VI  - 296
IP  - 5565
4099  - http://science.sciencemag.org/content/296/5565/141.short
4100  - http://science.sciencemag.org/content/296/5565/141.full
SO  - Science2002 Apr 05; 296
AB  - Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKENArabidopsis full-length (RAFL) cDNA clones. The 3′-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5′ ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.