RT Journal Article
SR Electronic
T1 Contribution of Human α-Defensin 1, 2, and 3 to the Anti-HIV-1 Activity of CD8 Antiviral Factor
JF Science
JO Science
FD American Association for the Advancement of Science
SP 995
OP 1000
DO 10.1126/science.1076185
VO 298
IS 5595
A1 Zhang, Linqi
A1 Yu, Wenjie
A1 He, Tian
A1 Yu, Jian
A1 Caffrey, Rebecca E.
A1 Dalmasso, Enrique A.
A1 Fu, Siyu
A1 Pham, Thang
A1 Mei, Jianfeng
A1 Ho, Jaclyn J.
A1 Zhang, Wenyong
A1 Lopez, Peter
A1 Ho, David D.
YR 2002
UL http://science.sciencemag.org/content/298/5595/995.abstract
AB It has been known since 1986 that CD8 T lymphocytes from certain HIV-1–infected individuals who are immunologically stable secrete a soluble factor, termed CAF, that suppresses HIV-1 replication. However, the identity of CAF remained elusive despite an extensive search. By means of a protein-chip technology, we identified a cluster of proteins that were secreted when CD8 T cells from long-term nonprogressors with HIV-1 infection were stimulated. These proteins were identified as α-defensin 1, 2, and 3 on the basis of specific antibody recognition and amino acid sequencing. CAF activity was eliminated or neutralized by an antibody specific for human α-defensins. Synthetic and purified preparations of α-defensins also inhibited the replication of HIV-1 isolates in vitro. Taken together, our results indicate that α-defensin 1, 2, and 3 collectively account for much of the anti–HIV-1 activity of CAF that is not attributable to β-chemokines.