PT  - JOURNAL ARTICLE
AU  - Festenstein, Richard
AU  - Pagakis, Stamatis N.
AU  - Hiragami, Kyoko
AU  - Lyon, Debbie
AU  - Verreault, Alain
AU  - Sekkali, Belaid
AU  - Kioussis, Dimitris
TI  - Modulation of Heterochromatin Protein 1 Dynamics in Primary Mammalian Cells
AID  - 10.1126/science.1078694
DP  - 2003 Jan 31
TA  - Science
PG  - 719--721
VI  - 299
IP  - 5607
4099  - http://science.sciencemag.org/content/299/5607/719.short
4100  - http://science.sciencemag.org/content/299/5607/719.full
SO  - Science2003 Jan 31; 299
AB  - Heterochromatin protein 1 (HP1β), a key component of condensed DNA, is strongly implicated in gene silencing and centromeric cohesion. Heterochromatin has been considered a static structure, stabilizing crucial aspects of nuclear organization and prohibiting access to transcription factors. We demonstrate here, by fluorescence recovery after photobleaching, that a green fluorescent protein–HP1β fusion protein is highly mobile within both the euchromatin and heterochromatin of ex vivo resting murine T cells. Moreover, T cell activation greatly increased this mobility, indicating that such a process may facilitate (hetero)chromatin remodeling and permit access of epigenetic modifiers and transcription factors to the many genes that are consequently derepressed.