PT - JOURNAL ARTICLE AU - Dietrich, Fred S. AU - Voegeli, Sylvia AU - Brachat, Sophie AU - Lerch, Anita AU - Gates, Krista AU - Steiner, Sabine AU - Mohr, Christine AU - Pöhlmann, Rainer AU - Luedi, Philippe AU - Choi, Sangdun AU - Wing, Rod A. AU - Flavier, Albert AU - Gaffney, Thomas D. AU - Philippsen, Peter TI - The <em>Ashbya gossypii</em> Genome as a Tool for Mapping the Ancient <em>Saccharomyces cerevisiae</em> Genome AID - 10.1126/science.1095781 DP - 2004 Apr 09 TA - Science PG - 304--307 VI - 304 IP - 5668 4099 - http://science.sciencemag.org/content/304/5668/304.short 4100 - http://science.sciencemag.org/content/304/5668/304.full SO - Science2004 Apr 09; 304 AB - We have sequenced and annotated the genome of the filamentous ascomycete Ashbya gossypii. With a size of only 9.2 megabases, encoding 4718 protein-coding genes, it is the smallest genome of a free-living eukaryote yet characterized. More than 90% of A. gossypii genes show both homology and a particular pattern of synteny with Saccharomyces cerevisiae. Analysis of this pattern revealed 300 inversions and translocations that have occurred since divergence of these two species. It also provided compelling evidence that the evolution of S. cerevisiae included a whole genome duplication orfusion of two related species and showed, through inferred ancient gene orders, which of the duplicated genes lost one copy and which retained both copies.