RT Journal Article SR Electronic T1 The Ashbya gossypii Genome as a Tool for Mapping the Ancient Saccharomyces cerevisiae Genome JF Science JO Science FD American Association for the Advancement of Science SP 304 OP 307 DO 10.1126/science.1095781 VO 304 IS 5668 A1 Dietrich, Fred S. A1 Voegeli, Sylvia A1 Brachat, Sophie A1 Lerch, Anita A1 Gates, Krista A1 Steiner, Sabine A1 Mohr, Christine A1 Pöhlmann, Rainer A1 Luedi, Philippe A1 Choi, Sangdun A1 Wing, Rod A. A1 Flavier, Albert A1 Gaffney, Thomas D. A1 Philippsen, Peter YR 2004 UL http://science.sciencemag.org/content/304/5668/304.abstract AB We have sequenced and annotated the genome of the filamentous ascomycete Ashbya gossypii. With a size of only 9.2 megabases, encoding 4718 protein-coding genes, it is the smallest genome of a free-living eukaryote yet characterized. More than 90% of A. gossypii genes show both homology and a particular pattern of synteny with Saccharomyces cerevisiae. Analysis of this pattern revealed 300 inversions and translocations that have occurred since divergence of these two species. It also provided compelling evidence that the evolution of S. cerevisiae included a whole genome duplication orfusion of two related species and showed, through inferred ancient gene orders, which of the duplicated genes lost one copy and which retained both copies.