RT Journal Article
SR Electronic
T1 Discovery and Directed Evolution of a Glyphosate Tolerance Gene
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1151
OP 1154
DO 10.1126/science.1096770
VO 304
IS 5674
A1 Castle, Linda A.
A1 Siehl, Daniel L.
A1 Gorton, Rebecca
A1 Patten, Phillip A.
A1 Chen, Yong Hong
A1 Bertain, Sean
A1 Cho, Hyeon-Je
A1 Duck, Nicholas
A1 Wong, James
A1 Liu, Donglong
A1 Lassner, Michael W.
YR 2004
UL http://science.sciencemag.org/content/304/5674/1151.abstract
AB The herbicide glyphosate is effectively detoxified by N-acetylation. We screened a collection of microbial isolates and discovered enzymes exhibiting glyphosate N-acetyltransferase (GAT) activity. Kinetic properties of the discovered enzymes were insufficient to confer glyphosate tolerance to transgenic organisms. Eleven iterations of DNA shuffling improved enzyme efficiency by nearly four orders of magnitude from 0.87 mM–1 min–1 to 8320 mM–1 min–1. From the fifth iteration and beyond, GAT enzymes conferred increasing glyphosate tolerance to Escherichia coli, Arabidopsis, tobacco, and maize. Glyphosate acetylation provides an alternative strategy for supporting glyphosate use on crops.