RT Journal Article SR Electronic T1 Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement? JF Science JO Science FD American Association for the Advancement of Science SP 1469 OP 1472 DO 10.1126/science.1108408 VO 308 IS 5727 A1 Kural, Comert A1 Kim, Hwajin A1 Syed, Sheyum A1 Goshima, Gohta A1 Gelfand, Vladimir I. A1 Selvin, Paul R. YR 2005 UL http://science.sciencemag.org/content/308/5727/1469.abstract AB We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)–tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be ∼8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.