RT Journal Article
SR Electronic
T1 Kinesin and Dynein Move a Peroxisome in Vivo: A Tug-of-War or Coordinated Movement?
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1469
OP 1472
DO 10.1126/science.1108408
VO 308
IS 5727
A1 Kural, Comert
A1 Kim, Hwajin
A1 Syed, Sheyum
A1 Goshima, Gohta
A1 Gelfand, Vladimir I.
A1 Selvin, Paul R.
YR 2005
UL http://science.sciencemag.org/content/308/5727/1469.abstract
AB We used fluorescence imaging with one nanometer accuracy (FIONA) to analyze organelle movement by conventional kinesin and cytoplasmic dynein in a cell. We located a green fluorescence protein (GFP)–tagged peroxisome in cultured Drosophila S2 cells to within 1.5 nanometers in 1.1 milliseconds, a 400-fold improvement in temporal resolution, sufficient to determine the average step size to be ∼8 nanometers for both dynein and kinesin. Furthermore, we found that dynein and kinesin do not work against each other in vivo during peroxisome transport. Rather, multiple kinesins or multiple dyneins work together, producing up to 10 times the in vitro speed.