RT Journal Article
SR Electronic
T1 MicroRNA Expression in Zebrafish Embryonic Development
JF Science
JO Science
FD American Association for the Advancement of Science
SP 310
OP 311
DO 10.1126/science.1114519
VO 309
IS 5732
A1 Wienholds, Erno
A1 Kloosterman, Wigard P.
A1 Miska, Eric
A1 Alvarez-Saavedra, Ezequiel
A1 Berezikov, Eugene
A1 de Bruijn, Ewart
A1 Horvitz, H. Robert
A1 Kauppinen, Sakari
A1 Plasterk, Ronald H. A.
YR 2005
UL http://science.sciencemag.org/content/309/5732/310.abstract
AB MicroRNAs (miRNAs) are small noncoding RNAs, about 21 nucleotides in length, that can regulate gene expression by base-pairing to partially complementary mRNAs. Regulation by miRNAs can play essential roles in embryonic development. We determined the temporal and spatial expression patterns of 115 conserved vertebrate miRNAs in zebrafish embryos by microarrays and by in situ hybridizations, using locked-nucleic acid–modified oligonucleotide probes. Most miRNAs were expressed in a highly tissue-specific manner during segmentation and later stages, but not early in development, which suggests that their role is not in tissue fate establishment but in differentiation or maintenance of tissue identity.