RT Journal Article
SR Electronic
T1 PIN Proteins Perform a Rate-Limiting Function in Cellular Auxin Efflux
JF Science
JO Science
FD American Association for the Advancement of Science
SP 914
OP 918
DO 10.1126/science.1123542
VO 312
IS 5775
A1 Petrášek, Jan
A1 Mravec, Jozef
A1 Bouchard, Rodolphe
A1 Blakeslee, Joshua J.
A1 Abas, Melinda
A1 Seifertová, Daniela
A1 Wiśniewska, Justyna
A1 Tadele, Zerihun
A1 Kubeš, Martin
A1 Čovanová, Milada
A1 Dhonukshe, Pankaj
A1 Skůpa, Petr
A1 Benková, Eva
A1 Perry, Lucie
A1 Křeček, Pavel
A1 Lee, Ok Ran
A1 Fink, Gerald R.
A1 Geisler, Markus
A1 Murphy, Angus S.
A1 Luschnig, Christian
A1 Zažímalová, Eva
A1 Friml, Jiří
YR 2006
UL http://science.sciencemag.org/content/312/5775/914.abstract
AB Intercellular flow of the phytohormone auxin underpins multiple developmental processes in plants. Plant-specific pin-formed (PIN) proteins and several phosphoglycoprotein (PGP) transporters are crucial factors in auxin transport–related development, yet the molecular function of PINs remains unknown. Here, we show that PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. Conditional gain-of-function alleles and quantitative measurements of auxin accumulation in Arabidopsis and tobacco cultured cells revealed that the action of PINs in auxin efflux is distinct from PGP, rate-limiting, specific to auxins, and sensitive to auxin transport inhibitors. This suggests a direct involvement of PINs in catalyzing cellular auxin efflux.