RT Journal Article
SR Electronic
T1 Whole-Genome Shotgun Sequencing of Mitochondria from Ancient Hair Shafts
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1927
OP 1930
DO 10.1126/science.1146971
VO 317
IS 5846
A1 Gilbert, M. Thomas P.
A1 Tomsho, Lynn P.
A1 Rendulic, Snjezana
A1 Packard, Michael
A1 Drautz, Daniela I.
A1 Sher, Andrei
A1 Tikhonov, Alexei
A1 Dalén, Love
A1 Kuznetsova, Tatyana
A1 Kosintsev, Pavel
A1 Campos, Paula F.
A1 Higham, Thomas
A1 Collins, Matthew J.
A1 Wilson, Andrew S.
A1 Shidlovskiy, Fyodor
A1 Buigues, Bernard
A1 Ericson, Per G. P.
A1 Germonpré, Mietje
A1 Götherström, Anders
A1 Iacumin, Paola
A1 Nikolaev, Vladimir
A1 Nowak-Kemp, Malgosia
A1 Willerslev, Eske
A1 Knight, James R.
A1 Irzyk, Gerard P.
A1 Perbost, Clotilde S.
A1 Fredrikson, Karin M.
A1 Harkins, Timothy T.
A1 Sheridan, Sharon
A1 Miller, Webb
A1 Schuster, Stephan C.
YR 2007
UL http://science.sciencemag.org/content/317/5846/1927.abstract
AB Although the application of sequencing-by-synthesis techniques to DNA extracted from bones has revolutionized the study of ancient DNA, it has been plagued by large fractions of contaminating environmental DNA. The genetic analyses of hair shafts could be a solution: We present 10 previously unexamined Siberian mammoth (Mammuthus primigenius) mitochondrial genomes, sequenced with up to 48-fold coverage. The observed levels of damage-derived sequencing errors were lower than those observed in previously published frozen bone samples, even though one of the specimens was >50,000 14C years old and another had been stored for 200 years at room temperature. The method therefore sets the stage for molecular-genetic analysis of museum collections.