RT Journal Article
SR Electronic
T1 Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1917
OP 1920
DO 10.1126/science.1151526
VO 318
IS 5858
A1 Yu, Junying
A1 Vodyanik, Maxim A.
A1 Smuga-Otto, Kim
A1 Antosiewicz-Bourget, Jessica
A1 Frane, Jennifer L.
A1 Tian, Shulan
A1 Nie, Jeff
A1 Jonsdottir, Gudrun A.
A1 Ruotti, Victor
A1 Stewart, Ron
A1 Slukvin, Igor I.
A1 Thomson, James A.
YR 2007
UL http://science.sciencemag.org/content/318/5858/1917.abstract
AB Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.