RT Journal Article SR Electronic T1 Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells JF Science JO Science FD American Association for the Advancement of Science SP 1917 OP 1920 DO 10.1126/science.1151526 VO 318 IS 5858 A1 Yu, Junying A1 Vodyanik, Maxim A. A1 Smuga-Otto, Kim A1 Antosiewicz-Bourget, Jessica A1 Frane, Jennifer L. A1 Tian, Shulan A1 Nie, Jeff A1 Jonsdottir, Gudrun A. A1 Ruotti, Victor A1 Stewart, Ron A1 Slukvin, Igor I. A1 Thomson, James A. YR 2007 UL http://science.sciencemag.org/content/318/5858/1917.abstract AB Somatic cell nuclear transfer allows trans-acting factors present in the mammalian oocyte to reprogram somatic cell nuclei to an undifferentiated state. We show that four factors (OCT4, SOX2, NANOG, and LIN28) are sufficient to reprogram human somatic cells to pluripotent stem cells that exhibit the essential characteristics of embryonic stem (ES) cells. These induced pluripotent human stem cells have normal karyotypes, express telomerase activity, express cell surface markers and genes that characterize human ES cells, and maintain the developmental potential to differentiate into advanced derivatives of all three primary germ layers. Such induced pluripotent human cell lines should be useful in the production of new disease models and in drug development, as well as for applications in transplantation medicine, once technical limitations (for example, mutation through viral integration) are eliminated.