RT Journal Article
SR Electronic
T1 High-Quality Binary Protein Interaction Map of the Yeast Interactome Network
JF Science
JO Science
FD American Association for the Advancement of Science
SP 104
OP 110
DO 10.1126/science.1158684
VO 322
IS 5898
A1 Yu, Haiyuan
A1 Braun, Pascal
A1 Yıldırım, Muhammed A.
A1 Lemmens, Irma
A1 Venkatesan, Kavitha
A1 Sahalie, Julie
A1 Hirozane-Kishikawa, Tomoko
A1 Gebreab, Fana
A1 Li, Na
A1 Simonis, Nicolas
A1 Hao, Tong
A1 Rual, Jean-François
A1 Dricot, Amélie
A1 Vazquez, Alexei
A1 Murray, Ryan R.
A1 Simon, Christophe
A1 Tardivo, Leah
A1 Tam, Stanley
A1 Svrzikapa, Nenad
A1 Fan, Changyu
A1 de Smet, Anne-Sophie
A1 Motyl, Adriana
A1 Hudson, Michael E.
A1 Park, Juyong
A1 Xin, Xiaofeng
A1 Cusick, Michael E.
A1 Moore, Troy
A1 Boone, Charlie
A1 Snyder, Michael
A1 Roth, Frederick P.
A1 Barabási, Albert-László
A1 Tavernier, Jan
A1 Hill, David E.
A1 Vidal, Marc
YR 2008
UL http://science.sciencemag.org/content/322/5898/104.abstract
AB Current yeast interactome network maps contain several hundred molecular complexes with limited and somewhat controversial representation of direct binary interactions. We carried out a comparative quality assessment of current yeast interactome data sets, demonstrating that high-throughput yeast two-hybrid (Y2H) screening provides high-quality binary interaction information. Because a large fraction of the yeast binary interactome remains to be mapped, we developed an empirically controlled mapping framework to produce a “second-generation” high-quality, high-throughput Y2H data set covering ∼20% of all yeast binary interactions. Both Y2H and affinity purification followed by mass spectrometry (AP/MS) data are of equally high quality but of a fundamentally different and complementary nature, resulting in networks with different topological and biological properties. Compared to co-complex interactome models, this binary map is enriched for transient signaling interactions and intercomplex connections with a highly significant clustering between essential proteins. Rather than correlating with essentiality, protein connectivity correlates with genetic pleiotropy.