PT  - JOURNAL ARTICLE
AU  - Geurts, Aron M.
AU  - Cost, Gregory J.
AU  - Freyvert, Yevgeniy
AU  - Zeitler, Bryan
AU  - Miller, Jeffrey C.
AU  - Choi, Vivian M.
AU  - Jenkins, Shirin S.
AU  - Wood, Adam
AU  - Cui, Xiaoxia
AU  - Meng, Xiangdong
AU  - Vincent, Anna
AU  - Lam, Stephen
AU  - Michalkiewicz, Mieczyslaw
AU  - Schilling, Rebecca
AU  - Foeckler, Jamie
AU  - Kalloway, Shawn
AU  - Weiler, Hartmut
AU  - Ménoret, Séverine
AU  - Anegon, Ignacio
AU  - Davis, Gregory D.
AU  - Zhang, Lei
AU  - Rebar, Edward J.
AU  - Gregory, Philip D.
AU  - Urnov, Fyodor D.
AU  - Jacob, Howard J.
AU  - Buelow, Roland
TI  - Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases
AID  - 10.1126/science.1172447
DP  - 2009 Jul 24
TA  - Science
PG  - 433--433
VI  - 325
IP  - 5939
4099  - http://science.sciencemag.org/content/325/5939/433.short
4100  - http://science.sciencemag.org/content/325/5939/433.full
SO  - Science2009 Jul 24; 325
AB  - The toolbox of rat genetics currently lacks the ability to introduce site-directed, heritable mutations into the genome to create knockout animals. By using engineered zinc-finger nucleases (ZFNs) designed to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38, we demonstrate that a single injection of DNA or messenger RNA encoding ZFNs into the one-cell rat embryo leads to a high frequency of animals carrying 25 to 100% disruption at the target locus. These mutations are faithfully and efficiently transmitted through the germline. Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.