RT Journal Article
SR Electronic
T1 Transnuclear Mice with Predefined T Cell Receptor Specificities Against <em>Toxoplasma gondii</em> Obtained via SCNT
JF Science
JO Science
FD American Association for the Advancement of Science
SP 243
OP 248
DO 10.1126/science.1178590
VO 328
IS 5975
A1 Kirak, Oktay
A1 Frickel, Eva-Maria
A1 Grotenbreg, Gijsbert M.
A1 Suh, Heikyung
A1 Jaenisch, Rudolf
A1 Ploegh, Hidde L.
YR 2010
UL http://science.sciencemag.org/content/328/5975/243.abstract
AB Mice that are transgenic for rearranged antigen-specific T cell receptors (TCRs) are essential tools to study T cell development and function. Such TCRs are usually isolated from the relevant T cells after long-term culture, often after repeated antigen stimulation, which unavoidably skews the T cell population used. Random genomic integration of the TCR α and β chain and expression from nonendogenous promoters represent additional drawbacks of transgenics. Using epigenetic reprogramming via somatic cell nuclear transfer, we demonstrated that T cells with predefined specificities against Toxoplasma gondii can be used to generate mouse models that express the TCR from their endogenous loci, without experimentally introduced genetic modification. The relative ease and speed with which such transnuclear models can be obtained holds promise for the construction of other disease models.