RT Journal Article
SR Electronic
T1 Cell types in the mouse cortex and hippocampus revealed by single-cell RNA-seq
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1138
OP 1142
DO 10.1126/science.aaa1934
VO 347
IS 6226
A1 Zeisel, Amit
A1 Muñoz-Manchado, Ana B.
A1 Codeluppi, Simone
A1 Lönnerberg, Peter
A1 La Manno, Gioele
A1 Juréus, Anna
A1 Marques, Sueli
A1 Munguba, Hermany
A1 He, Liqun
A1 Betsholtz, Christer
A1 Rolny, Charlotte
A1 Castelo-Branco, Gonçalo
A1 Hjerling-Leffler, Jens
A1 Linnarsson, Sten
YR 2015
UL http://science.sciencemag.org/content/347/6226/1138.abstract
AB The mammalian brain has an extraordinarily large number of cells. Although there are quite a few different cell types, many cells in any one category tend to look alike. Zeisel et al. analyzed the transcriptomes of mouse brain cells to reveal more than meets the eye. Interneurons of similar type were found in dissimilar regions of the brain. Oligodendrocytes that seemed to be all of one class were differentiated by their molecular signatures into a half-dozen classes. Microglia associated with blood vessels were distinguished from look-alike perivascular macrophages. Thus, the complex microanatomy of the brain can be revealed by the RNAs expressed in its cells.Science, this issue p. 1138The mammalian cerebral cortex supports cognitive functions such as sensorimotor integration, memory, and social behaviors. Normal brain function relies on a diverse set of differentiated cell types, including neurons, glia, and vasculature. Here, we have used large-scale single-cell RNA sequencing (RNA-seq) to classify cells in the mouse somatosensory cortex and hippocampal CA1 region. We found 47 molecularly distinct subclasses, comprising all known major cell types in the cortex. We identified numerous marker genes, which allowed alignment with known cell types, morphology, and location. We found a layer I interneuron expressing Pax6 and a distinct postmitotic oligodendrocyte subclass marked by Itpr2. Across the diversity of cortical cell types, transcription factors formed a complex, layered regulatory code, suggesting a mechanism for the maintenance of adult cell type identity.