RT Journal Article
SR Electronic
T1 Design of ordered two-dimensional arrays mediated by noncovalent protein-protein interfaces
JF Science
JO Science
FD American Association for the Advancement of Science
SP 1365
OP 1368
DO 10.1126/science.aaa9897
VO 348
IS 6241
A1 Gonen, Shane
A1 DiMaio, Frank
A1 Gonen, Tamir
A1 Baker, David
YR 2015
UL http://science.sciencemag.org/content/348/6241/1365.abstract
AB DNA has been used as a nano building material since the 1980s. Protein nanostructures have the potential to give greater geometric control and shape variability. Gonen et al. describe the computational design of proteins that self-assemble into two-dimensional arrays. These programmable protein lattices should enable new approaches in biomolecular structure determination and molecular sensing.Science, this issue p. 1365We describe a general approach to designing two-dimensional (2D) protein arrays mediated by noncovalent protein-protein interfaces. Protein homo-oligomers are placed into one of the seventeen 2D layer groups, the degrees of freedom of the lattice are sampled to identify configurations with shape-complementary interacting surfaces, and the interaction energy is minimized using sequence design calculations. We used the method to design proteins that self-assemble into layer groups P 3 2 1, P 4 21 2, and P 6. Projection maps of micrometer-scale arrays, assembled both in vitro and in vivo, are consistent with the design models and display the target layer group symmetry. Such programmable 2D protein lattices should enable new approaches to structure determination, sensing, and nanomaterial engineering.