Table 1.

Binding affinities (14) of peptides to Bcl-xL. Residues of Bak peptide substituted with alanine are in boldface. Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.

PeptideSequenceKD (μM)
Bak72 GQVGRQLAIIGDDINRRYDSEFQ 940.20 ± 0.02
72 GQVGRQLAIIGDDINR....... 870.34 ± 0.03
77 .....QLAIIGDDINR....... 87No binding
GQAGRQLAIIGDDINR.......15 ± 3
GQVGAQLAIIGDDINR.......3.3 ± 1
GQVGRQAAIIGDDINR.......270 ± 90
GQVGRQLAAIGDDINR.......1.0 ± 0.2
GQVGRQLAIAGDDINR.......17 ± 6
GQVGRQLAIIADDINR.......0.50 ± 0.1
GQVGRQLAIIGADINR.......41 ± 4
GQVGRQLAIIGDAINR.......0.14 ± 0.02
GQVGRQLAIIGDDANR.......93 ± 20
BcI-291 PVVHLALRQAGDDFSR....... 1066.4 ± 0.8
Bax57 KKLSECLKRIGDELDS....... 7213 ± 3
Bik55 DALALRLACIGDEMDV....... 7015 ± 6
Bcl-xL84 AAVKQALREAGDEFEL....... 99325