Table 1.

Two-hybrid interactions between Cdc42p and Bni1p. Assays were done as described (28) with strain Y704; pJG4-5 was the vector control.

DNA-binding domain fusionActivation-domain fusionlacZ expression (Miller units)
Cdc42pVector4.3 ± 0.2
Cdc42pBni1p(1-1953)17.0 ± 0.2
Cdc42pBni1p(1-1214)59.0 ± 2.0
Cdc42pBni1p(1215-1953)4.1 ± 0.2
Cdc42p(G12V)Vector16.0 ± 1.0
Cdc42p(G12V)Bni1p(1-1214)122.0 ± 7.0
Cdc42p(D118A)Vector0.2 ± 0.1
Cdc42p(D118A)Bni1p(1-1214)0.1 ± 0.0