Table 2.

Two-hybrid interactions among profilin, actin, Bni1p, and Bud6p. Assays were done as described (28) with strains Y704 (for assays involving either DBD or AD profilin constructs and for vector controls) and Y523 (remaining assays). For a given DBD fusion, the AD vectors gave indistinguishable basal levels of lexAop-lacZ reporter expression; therefore, only one control assay is presented in each case.

DNA-binding domain fusionActivation-domain fusionlacZ expression (Millerunits)
Pfy1pVector0.2 ± 0.1
Pfy1pAct1p132.0 ± 5.0
Pfy1pBni1p(1-1953)8.8 ± 1.0
Pfy1pBni1p(1-1214)0.2 ± 0.0
Pfy1pBni1p(1227-1397)64.0 ± 1.0
Pfy1pBni1p(1414-1953)0.2 ± 0.1
Pfy1pBni1p(1647-1953)0.5 ± 0.1
Bni1p(1227-1397)Vector0.4 ± 0.1
Bni1p(1227-1397)Pfy1p80.0 ± 2.0
Pfy1p-3Vector0.1 ± 0.0
Pfy1p-3Act1p127.0 ± 4.0
Pfy1p-3Bni1p(1227-1397)0.1 ± 0.0
Bni1p(1227-1397)Vector0.5 ± 0.1
Bni1p(1414-1953)Vector0.7 ± 0.2
Bni1p(1647-1953)Vector0.5 ± 0.0
Bni1p(1227-1397)Bud6p(478-788)0.5 ± 0.1
Bni1p(1414-1953)Bud6p(478-788)359.0 ± 54.0
Bni1p(1647-1953)Bud6p(478-788)296.0 ± 20.0
Bni1p(1227-1397)Act1p48.0 ± 0.3
Bni1p(1414-1953)Act1p0.4 ± 0.1
Bni1p(1647-1953)Act1p0.3 ± 0.1