Table 1

Fatty acid composition of S. typhimuriumLPS. LPS fatty acids were analyzed as their methyl esters by capillary GC with flame ionization detection (GC-FID) as described (22). The identities of the individual fatty acyl chains were confirmed by capillary GC with electron impact MS. Data shown are the average of three separate analyses (mean ± SD,n = 3). ND, not detected.

Fatty acidLPS (nmol/mg)
WT strainPhoPc strain PhoP strain
C12:0 46  ± 8 81  ± 9 37  ± 1
C14:0 43  ± 6 45  ± 536  ± 3
2-OH C14:0 1.0  ± 2 48  ± 1ND
3-OH C14:0 130  ± 20 236  ± 2598  ± 7
C16:0 57  ± 10 118  ± 2136  ± 8
Total 281  ± 42 532  ± 52 211  ± 5