Table 1

HIV-1–expressing cells in lymph nodes with and without opportunistic infections. Excisional biopsies were obtained with consent from 10 males, aged 28 to 50, and one 33-year-old female with AIDS-defining opportunistic infections (OIs) and from 67 HIV-1 seropositive subjects without evidence of OIs. Tissue sections were processed without and with (shown) protease and in situ hybridization using HIV-1 sense and antisense probes performed as described (7). After in situ hybridization, the entire tissue sections were imaged with a CCD camera and 60-mm macro lens. Using NIH Image and a Macintosh 8100/80AV computer, the areas of the sections were determined by planimetry based on a 10-mm bar standard. Subsequently, HIV-1 expressing mononuclear cells (not FDC associated HIV) in the entire tissue sections were counted in a darkfield microscope at a numerical aperture of 0.32. Positive cells were considered to have more than 20 silver grains per 200 μm2 and were expressed as the number of productively infected cells per 10 mm2 of lymph node tissue. Most of the cells in the OI-infected tissues had far more than this number of grains directly over the cell, making it impossible to quantitate their numbers. Cell numbers in highly infected tissues represent underestimates due to giant cells, the presence of multiple cell aggregation, and intense positivity of signal. MAC, Mycobacterium avium complex; MK, Mycobacterium kansasii; PC,Pneumocystis carinii; MTB, Mycobacterium tuberculosis; Histo, Histoplasma capsulatum.

PatientOIHIV-1+ cells per 10 mm2
Non-AIDS *
Follicular hyperplasia (n = 24)None2.8
Hyperplasia/involution (n = 21)None1.4
Follicular involution (n = 18)None2.0
Lymphocyte depleted (n = 4)None0.5
  • * Non-AIDS tissues include specimens and unpublished data from the Division of AIDS Treatment Research Initiative Study 003.