Data collection
| |

Resolution limit (Å) | 2.4 |

Total reflections (N) | 35,339 |

Unique reflections (N) | 15,811 |

Completeness (%) | 81 |

R_{sym}(%)^{*} | 6.6 |

Search model | ||
---|---|---|

GAP-334(718–1042) | H-Ras(1–166) | |

Molecular replacement
| ||

Resolution range (Å) | 15 to 4 | 15 to 4 |

Θ_{1}, Θ_{2}, Θ_{3}rotation^{†} (degrees) | 18.6, 36.8, 256 | 31.6, 89.8, 250.2 |

x, y, ztranslation^{‡} | 0.059, 0.0, 0.273 | 0.36, 0.0, 0.49 |

Refinement
| |

Resolution (Å) | 30 to 2.5 |

Number of unique reflections | 15,548 |

R_{cryst}(%)^{§} | 23.3 |

R_{free}(%)^{|} | 32.3 |

rmsd bond length (Å) | 0.007 |

rmsd bond angles (degrees) | 1.27 |

↵*

*R*_{sym}= Σ|*I*_{hi}−*I*_{h}|/Σ*I*_{hi}, where*I*_{hi}is the scaled intensity of the*i*th symmetry-related observation of reflection*h*and*I*_{h}is the mean value.↵† Eulerian angles Θ

_{1}, Θ_{2}, Θ_{3}, as defined in X-PLOR.↵‡ Fractional coordinates

*x, y, z*as obtained by X-PLOR.↵§

*R*_{cryst}= Σ_{h}|*F*_{oh}−*F*_{ch}|/*Σ*_{h}*F*_{oh}, where*F*_{oh}and*F*_{ch}are the observed and calculated structure factor amplitudes for reflection*h*.↵| Value of

*R*_{cryst}for 10% randomly chosen reflections not included in the refinement.