Table 1

Protective capacity of mAbs against VSV-IND. For details of the in vivo protection assay, see (19). The serum antibody concentration required for protection of 50% of passively immunized SCID mice (50% protective concentration) was identified by graphical extrapolation as illustrated in Fig. 2A, lower left panel, and division by the diffusion volume of a mouse (3 ml). For details of the in vitro analysis of antibodies, see (13). ND, could not be determined.

CloneSubclassIn vitroIn vivo
Avidity (M−1)Neutralization rate constant (M−1s−1)Neutralizing capacity (μg−1)*50% protective concentration (μg/ml)
VI22IgG2a9  ×  109 6  ×  106 1,2000.3
VI42IgG2a2.5  ×  107 ND0.9>30
VI41IgG2a2  ×  108 ND71
25G9IgG2a3  ×  109 2  ×  106 2910.3
VI46IgG2a2.5  ×  109 2  ×  105 565
VI24IgG2a5  ×  109 2  ×  107 3007
VI49IgG2a5.9  ×  109 4  ×  107 2,3603
VI30IgG2a2.2  ×  109 6  ×  106 1823
VI7IgG15  ×  109 7  ×  107 3,3000.3
VI29IgG12.5  ×  109 1  ×  107 5202
VI40IgG34  ×  108 9  ×  105 1770.3
G7G9E4IgM7  ×  109 7  ×  107 15,0001.4
G14H3D7IgM3  ×  109 ND10,4907
  • * Dilution of antibody with a starting concentration of 1 μg/ml yielding 50% plaque reduction in a standard neutralization assay.