Table 1

Angiogenic effect of vMIPs on the chick CAM.

Sample*Dose (μg)Number of embryosAngiogenic responseScorePercent maximum score
+±
vMIP-I2.096307.583
1.098108.594
0.56402467
0.252513481560
0.05174948.550
0.019225333
vMIP-II2.074305.579
1.0106317.575
0.584315.569
0.252481241458
0.05187569.553
0.018044225
hMIP-1α0.259027111
RANTES0.258125225
VEGF0.577007100
0.2563304.575
IL-8§ 1.091352.528
0.257025114
TPA0.0877007100
BSA20800800
H2O23022114
  • * Angiogenesis induction was performed by a methylcellulose disk containing synthetically prepared vMIP-I, vMIP-II, human MIP-1α, and RANTES. VEGF and TPA were used as positive controls. A disk containing vehicle only or 20 μg of BSA was used as a negative control.

  • Angiogenic responses were judged by three investigators: (+) unanimously positive; (±) unclear or split judgment; (−) unanimously negative.

  • Scores of 1, 0.5, and 0 were counted for (+), (±), and (−) results, respectively. Percent maximum score is the division of the score by the embryo number.

  • § A CXC ELR-containing chemokine.