Table 1

Percentages of phenotypes observed after injection of synthetic mRNAs.

Injected material (pg)nOne to three somites24 hours
Dorsalized*StrongdorsalizedWeakdorsalizedVentralized§
tolloid(tld) (100)56400095.2
tolloidMut (100)2674.51.94.80
tolloidMetallo (100)2370000
DN-BR (100)17987.183.23.30
DN-BR/tld(100/200)19791.482.27.10
noggin(nog; 20)21492.592.51.50
nog/tld(20/200)23393.192.33.80
chordin(chd; 100)32591.780.98.90
chd/tld(50/100)18722.48.017.629.4
chd/tld(50/200)1947.25.65.186.1
  • * As characterized by the rugby-ball shape, expanded notochord, and somites of dorsalized zebrafish mutants (27).

  • Judged to be equal to or more severe than the phenotype of the zebrafish snailhousemutation (27).

  • Judged to be equal to or less severe than the phenotype of the zebrafishpiggytail mutation (27).

  • § As characterized by expanded hematopoietic region and yolk extension similar to the ventralizing mutations chordino andmercedes (3, 5, 11).