Table 2

Sequence-specific and phosphorylation-dependent PPIase activity of Pin1. Assays were performed as described in Table 1, with the exception that trypsin replaced chymotrypsin as an isomer-specific protease when peptides with Pro-Arg-pNA sequences were used as substrates.

Substratekcat/Km (mM−1 s−1)
Suc-AAPL-pNA55
Suc-AAPR-pNA121
Suc-AAPM-pNA134
Suc-ADPY-pNA220
Suc-AEPF-pNA3,410
Ac-AYPY-pNA5
Ac-ApYPY-pNA3
Ac-ASPY-pNA269
Ac-ApSPY-pNA3,370
Ac-ATPY-pNA635
Ac-ApTPY-pNA2,480
AAEPF-pNA220
AASPF-pNA9
AApSPF-pNA3,760
AATPF-pNA4
AApTPF-pNA1,370
Ac-AASPR-pNA7
Ac-AApSPR-pNA9,300
Ac-AApTPR-pNA3,700
WYpSPRT-pNA14,100
WFYSPR-pNA170
WFYpSPR-pNA20,160