Table 3

Binding specificity of Pin1 and MPM-2. GST-Pin1 and MPM-2 were incubated with the pS-P–oriented degenerate peptide library H2N-MAXXXpSPXXAKK, where X is any amino acid except Cys. After extensive washing, peptides bound to GST-Pin1 or MPM-2 were eluted and sequenced. Amino acids selected at each degenerate position are shown.

LigandPosition
−3−2−10+1+2+3
Pin1WFYpSPRLX
YIRFI
FFY
WW
MPM-2YWFpSPLXX
FFLI
WIV
F
M